产品详情

  • 产品名称：Human ACHE (Acetylcholinesterase) Sandwich ELISA (STJE0004043)

  • 产品型号：STJE0004043
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ACHE (Acetylcholinesterase) Sandwich ELISA (STJE0004043)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38IU/mL
Detection Limit: 15.63~1000IU/mL
Short Description: This ACHE Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ACHE
Gene ID: 43
Uniprot ID: ACES_HUMAN
Specificity: This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Protein Name Acetylcholinesterase
Ache
Database Links Reactome: R-HSA-112311
Reactome: R-HSA-1483191
Reactome: R-HSA-422085
Cellular Localisation Synapse
Secreted
Cell Membrane
Peripheral Membrane Protein
Isoform T: Nucleus
Only Observed In Apoptotic Nuclei
Isoform H: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative ELISA Names Acetylcholinesterase ELISA kit
Ache ELISA kit
ACHE ELISA kit
Specificity This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 97
EDTA plasma (n=8) 97-107 100
Cell culture media (n=8) 85-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (IU/mL) 42.96 103.71 476.60 47.71 120.40 458.41
Standard deviation 2.20 5.05 23.83 2.84 5.78 22.55
CV (%) 5.12 4.87 5.00 5.95 4.80 4.92
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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