产品详情

  • 产品名称：Human FGFR1 alpha (IIIc) protein (Recombinant) (C-His-Avi) {Biotin} (STJP001740)

  • 产品型号：STJP001740
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human FGFR1 alpha (IIIc) protein (Recombinant) (C-His-Avi) {Biotin} (STJP001740)
详情介绍：
Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human FGFR1 alpha (IIIc)-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Immunogen Region: Arg22-Glu374
Immunogen: DNA sequence encoding Human FGFR1 alpha (IIIc) including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation. Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.
Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Protein Name Fibroblast Growth Factor Receptor 1
Fgfr-1
Basic Fibroblast Growth Factor Receptor 1
Bfgfr
Bfgf-R-1
Fms-Like Tyrosine Kinase 2
Flt-2
N-Sam
Proto-Oncogene C-Fgr
Cd Antigen Cd331
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839120
Reactome: R-HSA-1839122
Reactome: R-HSA-190370 P11362-19
Reactome: R-HSA-190373 P11362-1
Reactome: R-HSA-190374 P11362-1
Reactome: R-HSA-2219530
Reactome: R-HSA-375165 P11362-1
Reactome: R-HSA-445144 P11362-1
Reactome: R-HSA-5654219
Reactome: R-HSA-5654687
Reactome: R-HSA-5654688
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654726
Reactome: R-HSA-5655302
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8853336
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Nucleus
Cytoplasm
Cytosol
Cytoplasmic Vesicle
After Ligand Binding
Both Receptor And Ligand Are Rapidly Internalized
Can Translocate To The Nucleus After Internalization
Or By Translocations From The Endoplasmic Reticulum Or Golgi Apparatus To The Cytosol
And From There To The Nucleus
Alternative Protein Names Fibroblast Growth Factor Receptor 1 protein
Fgfr-1 protein
Basic Fibroblast Growth Factor Receptor 1 protein
Bfgfr protein
Bfgf-R-1 protein
Fms-Like Tyrosine Kinase 2 protein
Flt-2 protein
N-Sam protein
Proto-Oncogene C-Fgr protein
Cd Antigen Cd331 protein
FGFR1 protein
BFGFR protein
CEK protein
FGFBR protein
FLG protein
FLT2 protein
HBGFR protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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