产品详情

  • 产品名称：Anti-Acetyl-NFE2L2-Lys599 antibody (STJ97230)

  • 产品型号：STJ97230
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Anti-Acetyl-NFE2L2-Lys599 antibody (STJ97230)
详情介绍：
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Nuclear factor erythroid 2-related factor 2-Lys599 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFE2L2
Gene ID: 4780
Uniprot ID: NF2L2_HUMAN
Specificity: Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when acetylation at K599.
Immunogen: Synthesized acetyl-peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599.
Post Translational Modifications Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated. Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocations into the nucleus. Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.
Function Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Also plays an important role in the regulation of the innate immune response and antiviral cytosolic DNA sensing. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of pro-inflammatory signaling pathways like MyD88-dependent and -independent and TNF-alpha signaling. Suppresses macrophage inflammatory response by blocking pro-inflammatory cytokine transcription and the induction of IL6. Binds to the proximity of pro-inflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the NRF2-binding motif and reactive oxygen species level. Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses. Once activated, limits the release of pro-inflammatory cytokines in response to human coronavirus SARS-CoV-2 infection and to virus-derived ligands through a mechanism that involves inhibition of IRF3 dimerization. Also inhibits both SARS-CoV-2 replication, as well as the replication of several other pathogenic viruses including Herpes Simplex Virus-1 and-2, Vaccinia virus, and Zika virus through a type I interferon (IFN)-independent mechanism.
Protein Name Nuclear Factor Erythroid 2-Related Factor 2
Nf-E2-Related Factor 2
Nfe2-Related Factor 2
Nrf-2
Hebp1
Nuclear Factor - Erythroid Derived 2 - Like 2
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-9679191
Reactome: R-HSA-9707587
Reactome: R-HSA-9707616
Reactome: R-HSA-9755511
Reactome: R-HSA-9759194
Reactome: R-HSA-9762114
Reactome: R-HSA-9818025
Reactome: R-HSA-9818027
Reactome: R-HSA-9818028
Reactome: R-HSA-9818749
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Cytosolic Under Unstressed Conditions: Ubiquitinated And Degraded By The Bcr(Keap1) E3 Ubiquitin Ligase Complex
Translocates Into The Nucleus Upon Induction By Electrophilic Agents That Inactivate The Bcr(Keap1) E3 Ubiquitin Ligase Complex
Alternative Antibody Names Anti-Nuclear Factor Erythroid 2-Related Factor 2 antibody
Anti-Nf-E2-Related Factor 2 antibody
Anti-Nfe2-Related Factor 2 antibody
Anti-Nrf-2 antibody
Anti-Hebp1 antibody
Anti-Nuclear Factor - Erythroid Derived 2 - Like 2 antibody
Anti-NFE2L2 antibody
Anti-NRF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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